To catalog proteins present in complex biological fluids, such as BALF

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asked Jul 11 in General by kitten5garden (190 points)
The same biological processes were enriched compared with either human plasma proteome or total human genome <a href="http://www.chemscene.com/108321-42-2.html">108321-42-2 web</a> calculated proteome, suggesting an active enrichment of plasma proteins in the lung rather than passive capillary leak. niaid.nih.gov/david/ease.htm]Figure 1 (abstract P293)Conclusions The clinical parameters examined in this study do not accurately reflect the EVLW and should not be used as surrogates for it.P294 Different tidal volumes induce similar elevation of lung injury <a href="http://www.chemscene.com/39262-14-1.html">MedChemExpress Ginsenoside C-K</a> markers in animals sensitized to injury by previous anesthesia and surgeryP Dostal, V Cerny, M Senkerik, R Parizkova, H Zivna, P Zivny University Hospital Hradec Kralove, Czech Republic Critical Care 2008, 12(Suppl 2):P294 (doi: 10.1186/cc6515) Introduction Large tidal volumes and transpulmonary pressures play a central role in pathogenesis of ventilator-induced lung injury. Moderate tidal volumes are considered safe in healthy lungs [1]. Recent studies suggested that insults like endotoxin [2] or surgery [3] sensitize the lung to injury by priming for an exaggerated response to a second stimulus. Our aim was to investigate how animals sensitized to lung injury by previous anesthesia and surgery respond to mechanical ventilation (MV). Methods Twenty-two male adult Sprague awley rats instrumented surgically under ether anesthesia with vascular catheters on the previous day were anesthetized, tracheostomized and randomly allocated to two mechanically ventilated groups ?MVLP group (FiO2 1.0, respiratory rate 60/min, tidal volume 10 ml/kg, PEEP 2 cmH2O, n = 8) and HVZP group (FiO2 1.0, respiratory rate 20/min, tidal volume 30 ml/kg, PEEP 0 cmH2O, n = 8) ?and a no MV group C (n = 6). After randomization (C group) or after 2 hours of MV, rats were sacrificed, the P  curve of the respiratory system constructed, and bronchoalveolar lavage fluid (BALF) and aortic blood samples obtained. Results Comparison of P  curves suggested derecruitment of the lung in the MVLP group, but no significant difference in <a href="http://www.ncbi.nlm.nih.gov/pubmed/28110187" title=View Abstract(s)">PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/28110187</a> airway pressures at maximal lung volume (14 ml) was observed between groups. Total protein (g/ml) in BALF was similar in both the MVLP and HVZP groups (0.21 (0.16, 0.30) and 0.22 (0.18, 0,50), P = 0.366) and lower in the C group (0.13 (0.07, 0.16), P < 0.05) versus both MV groups. Similar results were obtained for IL-6 levels (pg/ml) in BALF (102.0 (93.4, 111.8) and 120.0 (53.7, 130.0) for the MVLP and HVZP groups, P = 0.628 and 48.5 (43.4, 56.4) pg/ml for the C group, P < 0.05 vs both MV groups). Conclusions Both moderate and high tidal volumes induce a similar elevation of lung injury markers in mechanically ventilated animals sensitized to injury by previous anesthesia and surgery.To catalog proteins present in complex biological fluids, such as BALF, from which hypotheses can be developed and tested. From the normal BALF proteome data, gene ontology analysis demonstrated enrichment of several biological processes in the lung that reflects its expected role in gas exchange and host defense as an immune organ. The same biological processes were enriched compared with either human plasma proteome or total human genome calculated proteome, suggesting an active enrichment of plasma proteins in the lung rather than passive capillary leak. References 1. Schnapp et al.: Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury.

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